2016 Fiscal Year Final Research Report
Study on the interaction between target cells and polyphenol conjugates underlying the molecular mechanism of their biological activity
| Project/Area Number |
26292069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | The University of Tokushima (2015-2016)
Nagoya University (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ポリフェノール / 抱合体 / マクロファージ / 標的細胞 / 標的分子 / 炎症 |
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| Outline of Final Research Achievements |
We first examined the anti-inflammatory effects of a quercetin glucuronide (Q3GA) and a sulfate (Q3'S) in macrophages. Next, to identify the target molecules of quercetin, a major polyphenol in human diet, and of its glucuronide metabolite (Q3GA), the derivatives of quercetin and Q3GA were chemically synthesized. We also developed a reaction condition for the formation of cross-link between proteins and these quercetin derivatives. On the other hand, we developed an analytical condition of LC-MS/MS for detection of quercetin metabolites in human plasma. High-sensitive LC-MS/MS analysis enables us to investigate the effects of different methods for quercetin intake on the metabolic profiles of quercetin in humans.
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| Free Research Field |
食品機能学
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