Strategic Design of Biointerfaces by Wood Carbohydrates for Cell Culture Applications

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292096
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Kyushu University
• Principal Investigator: KITAOKA Takuya 九州大学, 大学院農学研究院, 教授 (90304766)
• Co-Investigator(Renkei-kenkyūsha): ICHINOSE Hirofumi 九州大学, 大学院農学研究院, 准教授 (00432948)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 糖鎖 / 自己組織化 / ナノ材料 / バイオインターフェース / 細胞培養 / 分化制御 / シグナル伝達 / バイオマテリアル

Outline of Final Research Achievements

Cell culture technology in vitro has recently attracted much attention in regenerative medicine. Herein, we propose the structural and biofunctional design of glyco-based cell culture scaffolds by using cello-/chito-oligosaccharides and/or crystalline polysaccharide nanofibers. In this project, we have completed the following achievements: (1) cell alignment and fusion of mouse myoblasts on self-assembled GlcNAc6-monolayers with various geometries, (2) promotion of the cell attachment of mouse fibroblasts NIH-3T3 on surface-carboxylated cellulose nanofibers, and (3) activation of the immune response of TLR2-expressing HEK293 cells on chitin nanofibers. Wood carbohydrates have potentials to act both as building blocks and bio-ligands for cell culture applications. Our challenge will open up a new phase of “Glyconanoarchitectonics”, based on the functional manipulation of cellular tissues by glyco-biointerface materials.

Free Research Field

木質科学・多糖材料化学