Identification of monolignol trasporter targeting ligninmodification in plant cell walls for the biomass production

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292097
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Kyushu University
• Principal Investigator: TSUTSUMI YUJI 九州大学, (連合)農学研究科(研究院), 教授 (30236921)
• Co-Investigator(Kenkyū-buntansha): 藤田 弘毅 九州大学, (連合)農学研究科(研究院), 助教 (90264100)
• Co-Investigator(Renkei-kenkyūsha): SHIGETO JUN 九州大学, 大学院・農学研究院, 特任助教 (70570852)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リグニンモノマー / 輸送体 / 細胞壁形成 / 培養細胞 / 分化誘導 / 発現解析 / リアルタイムPCR / 統計解析

Outline of Final Research Achievements

The candidate genes for extracellular monolignol transport were screened. Tracheary element (TE) induced cells in which lignification is promoted was employed. Besides, different plant organ samples, which has different lignification were also applied. The expression analysis of the transporter genes in these samples was performed. Secondary wall formation and lignin synthesis related genes were also subjected to the analysis as references. Expression profile with TE differentiated samples showed four transporter genes highly expressed synchronously with reference genes. Besides, five transporter genes from plant organ analysis were also screened as candidates. There was no significant change in the amount of lignin in the single gene knockout mutant of the candidates. The expression levels of two other candidates were largely promoted in the single gene knockout plant, suggesting that these transporter candidates were functionally redundant.

Free Research Field

木質科学