Development of new aquaculture using designated nuclease

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292104
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Kyoto University
• Principal Investigator: Kinoshita Masato 京都大学, (連合)農学研究科(研究院), 助教 (60263125)
• Co-Investigator(Kenkyū-buntansha): 豊田 敦 国立遺伝学研究所, 大学共同利用機関等の部局等, その他 (10267495) ; 家戸 敬太郎 近畿大学, 付置研究所, 教授 (90330240) ; 吉浦 康寿 国立研究開発法人水産研究・教育機構, その他部局等, 研究員 (90372052)
• Research Collaborator: KISHIMOTO Kenta 京都大学, 大学院農学研究科 ; Murakami Yu 京都大学, 大学院農学研究科 ; KATAYAMA Takashi 国立研究開発法人水産研究, 教育機構 ; WASHIO Yohei 近畿大学, 水産研究所
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゲノム編集 / マダイ / トラフグ / ミオスタチン / CRISPR/Cas9

Outline of Final Research Achievements

In aquaculture, selective breeding has not developed yet. The selective breeding takes much time to establish a new breed, so it is not adequate for aquaculture where new breeds are required immediately. We tried to develop new breeding method for aquaculture using genome editing technology, CRISPR/Cas9. With red sea bream and tiger puffer, the single guide RNAs were designated on the genome sequence in exon 1 of myostatin gene and microinjected into the one-cell stage eggs which are artificially inseminated. As results, the myostain gene of both fish has successfully disrupted and new breeds with enhanced muscle mass have established.

Free Research Field

魚類発生工学