Elucidation of embryonic secretory factors using bovine endometrial spheroids

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292141
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyushu University
• Principal Investigator: Yamauchi Nobuhiko 九州大学, (連合)農学研究科(研究院), 准教授 (00363325)
• Co-Investigator(Kenkyū-buntansha): 川原 学 北海道大学, (連合)農学研究科(研究院), 准教授 (70468700)
• Research Collaborator: Md. Rashedul Islam ; Mohamed El-Sharawy
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ウシ / 子宮内膜 / スフェロイド / 胚性分泌因子 / RNAseq / LC/MS/MS

Outline of Final Research Achievements

The present study aimed to analyze genes whose expression is induced by embryos and to identify embryonic secretory factors that induce gene expression in cattle. Analysis of the embryonic secretory factor of bovine elongated embryo by LC / MS / MS resulted in the identification of 6 cytokines and 2 growth factors. Furthermore, from the results obtained by RNAseq analysis, upstream factors were analyzed for genes whose expression increased in the endometrium of the implantation period. As a result, in addition to IFNT, genes considered to be controlled by MIF was included. These results suggested that MIF might control endometrial function during implantation as an embryonic secretory factor other than IFNT.

Free Research Field

動物繁殖生理学