2016 Fiscal Year Final Research Report
Elucidation of the molecular mechanism for pathogenesis by avian rotavirus using a reverse genetics approach
| Project/Area Number |
26292148
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 直人 岐阜大学, 応用生物科学部, 准教授 (20334922)
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| Research Collaborator |
OKADARA Kota
MITAKE Hiromichi
OKADA Kazuma
NAKAGAWA Kento
TAMADA Daigo
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 鳥ロタウイルスA / 遺伝的多様性 / 遺伝子操作系 |
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| Outline of Final Research Achievements |
This research project was conducted to reveal molecular mechanisms by which avian rotavirus A (RAV) induces pathogenicity in mammals. Firstly, we analyzed genome sequences from 3 avian RAVs and compared them with sequences of avian RAVs from various species. We discovered unique genome sequences in the 2 RAVs, which were certificated as novel genotypes by the Rotavirus Classification Working Group. Next, we tried to establish a reverse genetics system for the avian RAV PO-13 strain to explore its pathogenicity to mammals. We constructed the reverse genetics system using T7 and polII promoter for synthesis of virus RNAs. Neither of the systems resulted in virus rescue. We, however, detected RAV specific replicon activity in the polII promoter system, indicating that the replication and transcription by the RAV polymerase could be induced artificially.
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| Free Research Field |
人獣共通感染症
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