Analysis and function enhancement of plant vesicle transport system as the basis of plant biomass production control

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292194
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyushu University
• Principal Investigator: Matsuoka Ken 九州大学, (連合)農学研究科(研究院), 教授 (40222294)
• Research Collaborator: TOYOOKA Kiminori
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゴルジ装置 / 高等植物 / 小胞輸送 / 機能変換 / 形質転換植物 / 栄養欠乏

Outline of Final Research Achievements

Cell wall polysaccharides such as pectin are known as major plant biomass. These are synthesized at the Golgi apparatus in the cell and are transported outside the cell via a vesicular transport system structure such as trans Golgi network (TGN).However the functional details and whether their function is controlled by nutrition had not been clarified.
Therefore, functional analysis of the Golgi apparatus was conducted mainly about the modification of glycoprotein. Analysis of structural transformation mechanism by TGN under sugar deficiency was also promoted. We also analyzed the localization and membrane orientation of proteins found in isolated fraction enriched with secretory vesicle cluster, which is one of the vesicular transport systems after TGN. These analyzes have deepened the accumulation of knowledge concerning the functions and control of the Golgi apparatus in plant cells.

Free Research Field

分子細胞生物学、応用植物生化学