Intracellular signaling machinery controlling endocytosis and uptake of extracellular materials

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Hokkaido University
• Principal Investigator: Ohba Yusuke 北海道大学, 医学(系)研究科(研究院), 教授 (30333503)
• Co-Investigator(Renkei-kenkyūsha): Nanbo Asuka 北海道大学, 大学院医学研究科, 准教授 (60359487) ; Nishide Shinya 北海道大学, 大学院医学研究科, 助教 (40451398) ; Fujioka Yoichiro 北海道大学, 大学院医学研究科, 助教 (70597492)
• Research Collaborator: Fujioka Mari ; Horiuchi Kosui ; Satoh Aya O.
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 蛍光バイオイメージング / エンドサイトーシス / シグナル伝達

Outline of Final Research Achievements

Endosome is a multifunctional platform through the emission and the regulation of a variety of signal transduction pathways. However, the relationship between signaling dynamics and cell function via the endosomes in living cells has yet to be analyzed in detail. In this study, we demonstrated that angiotensin II (AII) type 2 receptor (AT2R) negatively regulates type 1 receptor (AT1R)-signaling through a series of events including heterodimerization of AT1R and AT2R, internalization via endocytosis of the complex, change in the molecular orientation of receptors in the complex. We also clarified that activation of both receptors and and phosphorylation of serine residues at the C-terminus of AT2R by protein kinase C (PKC) are indispensable for this function.

Free Research Field

細胞生理学