Analyses of an orphan metabotropic receptor which plays a role in the long term retention of memory, and a secretory protein encoded by its splice variant

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: KUBO Yoshihiro 生理学研究所, 分子細胞生理研究領域, 教授 (80211887)
• Co-Investigator(Kenkyū-buntansha): 今野 幸太郎 北海道大学, 医学(系)研究科(研究院), 助教 (20599641)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Izumi 生理学研究所, 分子生理研究系, 特別研究員 (20645254) ; WATANABE Masahiro 北海道大学, 医学(系)研究科(研究院), 教授 (70210945)
• Research Collaborator: Chen I-Shan 生理学研究所, 特任助教 ; Yamamoto Tomomi 生理学研究所, 技術職員 ; TATEYAMA Michihiro 生理学研究所, 准教授 ; TAKAO Keizo 生理学研究所, 特任准教授 ; MIYAKAWA Tsuyoshi 藤田保健衛生大学, 教授 ; HATTORI Satoko 藤田保健衛生大学, 助教 ; FUKATA Masaki 生理学研究所, 教授 ; FUKATA Yuko 生理学研究所, 准教授 ; YOKOI Norihiko 生理学研究所, 助教 ; SAKIMURA Kenji 新潟大学, 脳研究所, 教授 ; SYUU Rei 新潟大学, 脳研究所, ポスドク研究員 ; NATSUME Rie 新潟大学, 脳研究所, 技術職員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Prrt3 / オーファン受容体 / スプライス異型 / 恐怖条件付け記憶 / 長期保持

Outline of Final Research Achievements

Prrt3 is a putative G protein coupled receptor of family C, but its function is totally unknown. We aimed at the elucidation of the molecular function and physiological role of Prrt3, and observed the followings. (1) Prrt3 is partly cleaved at the end of N-terminal extracellular domain by Furin. (2) It binds to glutamate transporters EAAT1, 2 and Gi/o G protein. (3) It is expressed chiefly at the presynaptic terminal of excitatory neurons. (4) Conditional Prrt3 gene knock out homozygous mice in forebrain excitatory neurons showed impairment of long term retention of fear conditioning memory. (5) Prtt3 is activated by Oxo-M, a muscarinic Ach receptor agonist, but not by ACh.

Free Research Field

分子生理学