Elucidation of molecular mechanisms of protease secretion in Entamoeba histolytica

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293093
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Nozaki Tomoyoshi 国立感染症研究所, 寄生動物部, 部長 (60198588)
• Co-Investigator(Kenkyū-buntansha): 津久井 久美子 国立感染症研究所, その他部局等, 研究員 (00420092) ; 中野 由美子 (斉藤由美子) 国立感染症研究所, その他部局等, 研究員 (30321764) ; 千葉 洋子 筑波大学, 生命環境科学研究科(系), 助教 (70638981)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 感染症 / 原虫 / 病原機構 / 膜輸送 / 代謝 / 創薬 / オルガネラ / 進化

Outline of Final Research Achievements

The enteric protozoan parasite Entamoeba histolyitca relies on Rab11B small GTPase-mediated vesicular traffic for its pathogenesis, more specifically secretion of cysteine proteases. We attempted to identify proteins that bind and regulate or are regulated by Rab11B. Three proteins, beta adaptin, gamma adaptin, and Sec6, among others, were identified as Rab11B binding proteins. These data indicate that Rab11B are involved in the processes encompassing vesicular budding through plasma membrane tethering. Our results helps in theory to formulate methods to prevent cysteine protease secretion and resulting tissue destruction by the amebae.

Free Research Field

感染症、原虫、病原機構、膜輸送、代謝、創薬、オルガネラ、進化