Energy transduction mechanism of the bacterial flagellar protein export apparatus

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293097
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: MINAMINO Tohru 大阪大学, 生命機能研究科, 准教授 (20402993)
• Co-Investigator(Renkei-kenkyūsha): IMADA Katsumi 大阪大学, 大学院理学研究科, 教授 (40346143)
• Research Collaborator: KINOSHITA Miki 大阪大学, 大学院生命機能研究科, 特任研究員 ; INOUE Yumi 大阪大学, 大学院生命機能研究科, 特任技術職員 ; MORIMOTO Yusuke 大阪大学, 大学院生命機能研究科, 招聘研究員 ; FUKUMURA Takuma 大阪大学, 大学院生命機能研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 電子顕微鏡 / 遺伝学 / 遺伝学 / 細菌 / 蛋白質

Outline of Final Research Achievements

The bacterial flagellar protein export apparatus, which consists of an export gate complex made of five transmembrane proteins and a cytoplasmic ATPase composed of three cytoplasmic proteins, utilizes ATP and proton motive force across the cytoplasmic membrane as the energy source to drive flagellar protein export. To clarify the energy transduction mechanism of the export apparatus, we have shown that the flagellar export apparatus is a fuel-efficient export engine that can save ATP consumption for the enormous amount of protein export for flagellar assembly and that FlhA acts as an ion channel of the export gate complex to couple the proton flow with protein transport.

Free Research Field

分子生物学・生物物理学