2016 Fiscal Year Final Research Report
Developmdent of BCG vaccines based on ehausted T cell re-activation
| Project/Area Number |
26293098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細菌学 / BCG / IL-21 / IL-7 / ワクチン / T細胞 |
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| Outline of Final Research Achievements |
Mycobacterium bovis Bacillus Calmette-Guerin (BCG) vaccine confers incomplete protection against tuberculosis because it is not effective for inducing long-term immunity. Therefore, it is urgently required to develop improved vaccines. We found that IL-7 and IL-21 are pleiotropic common cytokines that affect IL-17+γδT cells and CD8+T cells, respectively. Based on these results, we examined the effects of recombinant BCG secreting fusion protein Ag85B/IL-7, or IL-21 (rBCG-Ag85B-IL7, rBCG-Ag85B-IL21) on cell-mediated immune responses against BCG. The levels of effector CD8+ T cells were significantly higher butexhausted CD8+ T cells were lower after immunization with rBCG-Ag85B-IL21 compared with rBCG-Ag85B. The levels of IL-17+γδ T cells and CD4+ Th1 cells were higher in rBCG-Ag85B-IL7-immunized mice than rBCG-Ag85B-immunized mice. rBCG-Ag85B-IL-7 or IL-21 vaccination capable of inducing long-term efficient immunity might be used as an effective vaccine for tuberculosis.
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| Free Research Field |
医歯薬学
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