2016 Fiscal Year Final Research Report
Molecular mechanism of viral RNP recognition by RIG-I-like receptors
| Project/Area Number |
26293101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
西城 忍 千葉大学, 真菌医学研究センター, 准教授 (60396877)
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| Co-Investigator(Renkei-kenkyūsha) |
ONOMOTO Koji 千葉大学, 真菌医学研究センター, 助教 (10612202)
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| Research Collaborator |
JOGI Michihiko
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ウイルス / 免疫学 / 核酸 / RNA結合タンパク質 |
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| Outline of Final Research Achievements |
In order to understand how viral RNA sensors, RIG-I-like receptors (RLRs), recognize viral ribonucleoprotein complexes (RNPs) in virus-infected cells, we assessed activation of RIG-I-mediated signaling by viral RNPs using in vitro reconstitution assay system. As a model RNPs, we prepared artificial RNPs of influenza A virus (IAV). In vitro assay indicated that RIG-I-mediated signaling is activated by IAV RNPs, however the data also suggest that host factor(s) is required for full-activation of the signal. The observations would help us to reveal a molecular machinery of activation of anti-viral innate immune responses.
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| Free Research Field |
分子生物学
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