Development of a novel oncolytic virus by regulating the processing of virus-derived non-coding RNA

2017 Fiscal Year Final Research Report

• Project/Area Number: 26293118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: Osaka University
• Principal Investigator: SAKURAI FUMINORI 大阪大学, 大学院薬学研究科, 准教授 (70370939)
• Co-Investigator(Renkei-kenkyūsha): MIZUGUCHI HIROYUKI 大阪大学, 大学院薬学研究科, 教授 (50311387) ; TACHIBANA MASASHI 大阪大学, 大学院薬学研究科, 特任准教授 (80513449)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: アデノウイルス / 非コードRNA / 腫瘍溶解性ウイルス / Dicer

Outline of Final Research Achievements

An adenovirus (Ad) genome encodes two non-coding RNAs, VA-RNA I and VA-RNA II. Previous studies demonstrated that VA-RNAs enhance Ad infection and that Dicer processes VA-RNAs, producing miRNA-like small RNAs (mivaRNA); however, relationship between Dicer-mediated processing of VA-RNAs and VA-RNA-mediated enhancement of Ad infection remained to be elucidated. In this study, we examined the effects of Dicer-mediated processing of VA-RNAs on Ad infection. We demonstrated that Dicer-mediated proessing of VA-RNA I resulted in loss of promotion activity of VA-RNA I for Ad infection. Based on these findings, we developed a novel oncolytic Ad showing superior antitumor effects.

Free Research Field

遺伝子治療学