Regenerative medicine using dedifferentiated fat cell-derived condition media and exosome

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293170
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: Nihon University
• Principal Investigator: MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580)
• Co-Investigator(Kenkyū-buntansha): 副島 一孝 日本大学, 医学部, 准教授 (00246589) ; 加野 浩一郎 日本大学, 生物資源科学部, 教授 (80271039) ; 風間 智彦 日本大学, 医学部, 助手 (80525668)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脱分化脂肪細胞 / 細胞治療 / 間葉系幹細胞 / 再生医療 / エクソソーム

Outline of Final Research Achievements

In the present study, we examined the characteristics and therapeutic potential of dedifferentiated fat (DFAT) cell-derived exosomes. As results, we found that DFAT cell-derived exosomes contained a variety of microRNAs that are thought to play important roles in intercellular communications. The expression profile of microRNAs in DFAT cell-derived exosomes was very similar to that in adipose-derived stem cell (ASC)-derived exosomes. DFAT cell-derived exosomes inhibited proliferation of T lymphocytes and promoted differentiation of naive T cells into regulatory T cells. In addition, DFAT cell-derived exosomes stimulated proliferation of nucleus pulposus cells of intervertebral disc and increased expression of SOX9, a critical transcription factor for chondrogenesis. These results suggest that DFAT cell-derived exosomes have therapeutic potential for T cell-mediated autoimmune disorders and intervertebral disc degeneration.

Free Research Field

再生医学　血管生物学