2016 Fiscal Year Final Research Report
Development of a novel high sensitivity molecular imaging for ACF
| Project/Area Number |
26293176
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐野 茂樹 徳島大学, 大学院医歯薬学研究部, 教授 (20226038)
中川 忠彦 徳島大学, 大学院医歯薬学研究部, 特任助教 (40634275)
六車 直樹 徳島大学, 大学院医歯薬学研究部, 准教授 (90325283)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Michiyasu 徳島大学, 大学院医歯薬学研究部, 助教 (60550001)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | molecular imaging / ACF / 蛍光標識プローブ |
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| Outline of Final Research Achievements |
We analyzed aberrant crypt foci (ACF) tissues using microarray to find a specific molecule for molecular imaging of ACF. Then, we found Glutathione S-transferase(GST)-pi、EGFR, glut-1 are overexpressed in ACF as a specific molecule for ACF molecular imaging. Synthesizing fluorescent probe targeting GST-pi, EGFR and glut-1, we clearly identified ACF on the colorectal mucosa in azxymethane-injected mouse model under fluorescence microscope. We also clearly visualized human ACF on colorectal mucosa resected from patients with colorectal cancers under fluorescence microscope. Thus, we were able to detect human and rodent precancerous ACF lesions using molecular imaging technique.
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| Free Research Field |
Gastroenterology
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