Establishment of Gene Engineered Organoids for Functional Analysis of Human Colorectal Driver Gene Mutations

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293177
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: Sato Toshiro 慶應義塾大学, 医学部, 准教授 (70365245)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 大腸癌 / 浸潤 / 転移 / オルガノイド / 幹細胞

Outline of Final Research Achievements

Recent advance in sequence technology has enabled comprehensive analyses of genetic mutations in human colorectal cancer. In this study, we combined organoid culture technology and CRISPR-Cas9 technology to establish gene engineered organoids for analysis of genetic mutations in human colorectal cancers. The engineered organoids were xenotransplanted into immune-deficient mice and pathologically examined for their invasive and metastatic capacity. The functional analysis platform for driver genetic mutations are instrumental and holds a promise for applying to drug screening. 

Free Research Field

消化器内科学