2017 Fiscal Year Final Research Report
Elucidation of hepatitis virus infection / replication mechanism using genome-modified human pluripotent stem cells
| Project/Area Number |
26293178
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAKINUMA Sei 東京医科歯科大学, 医学部, 准教授 (30372444)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | ヒトiPS細胞 |
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| Outline of Final Research Achievements |
Chronic infection of hepatitis B virus (HBV, HCV) is a major cause of cirrhosis, liver cancer etc in Japan. However, it was difficult to analyze because of the species specificity of its infectivity. In this study, for the purpose of deriving hepatocytes from human iPS cells and constructing an HBV infection analysis system, 1) preparation of highly expressed human hepatocytes of HBV receptors, 2) genes responsible for high function of hepatocytes Screening, etc. were conducted. As a result, it has been found that HBV is efficiently infected / amplified in human iPS cell-derived hepatocytes overexpressing Ntcp, and that KLF 15 is important for enhancing hepatocyte function.
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| Free Research Field |
肝細胞生物学
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