MHC-matched allogeneic transplantation of iPS cell-derived cardiomyocytes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293182
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Shinshu University
• Principal Investigator: SHIBA Yuji 信州大学, 医学部附属病院, 准教授(特定雇用) (70613503)
• Co-Investigator(Kenkyū-buntansha): 瀬戸 達一郎 信州大学, 学術研究院医学系(医学部附属病院), 講師 (70362118)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 循環器内科学 / 再生医学

Outline of Final Research Achievements

iPS cells were generated from fibroblasts from an animal with a homozygous MHC haplotype (HT4), and the cells were differentiated into cardiomyocytes (iPSC-CMs). Five HT4 heterozygous monkeys were subjected to myocardial infarction followed by direct intramyocardial injection of the iPSC-CMs. The grafted cardiomyocytes survived for 12 weeks with no evidence of immune rejection, and they showed indisputable electrical integration with host cardiomyocytes. In addition, transplantation of the iPSC-CMs improved cardiac contractile function; however, the incidence of VT was transiently but significantly increased when compared to the vehicle-treated control. MHC-matched allogeneic transplantation of iPSC-CMs regenerates infarcted non-human primate heart. Further research to control post-transplant arrhythmias is warranted.

Free Research Field

再生医学