Therapeutic angiogenesis using autologous adipose-derived regenerative cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293184
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Nagoya University
• Principal Investigator: MUROHARA TOYOAKI 名古屋大学, 医学(系)研究科(研究院), 教授 (90299503)
• Co-Investigator(Kenkyū-buntansha): 新谷 理 名古屋大学, 医学(系)研究科(研究院), 寄附講座准教授 (20309777) ; 柴田 玲 名古屋大学, 医学(系)研究科(研究院), 寄附講座准教授 (70343689) ; 近藤 和久 名古屋大学, 医学(系)研究科(研究院), 助教 (90644659)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 再生医療 / 血管再生療法 / 幹細胞 / 四肢虚血 / 脂肪組織由来幹細胞 / 血管内皮細胞 / 皮下脂肪組織 / サイトカイン

Outline of Final Research Achievements

Our final goal of this research series is to establish the clinical therapeutic angiogenesis in patients with critical limb ischemia manifestating rest pain and/or ischemic skin ulcer due to atherosclerotic disease, Burger's disease and connective tissue diseases such as scleroderma (PSS). To this end, we have established mouse, rat and rabbit model of hind limb ischemia, and we examined the effects of the implantation of autologous adipose-tissue derived regenerative cells (ADRCs), one of the mesenchymal stem cell class, on limb blood perfusion and angiogenesis in these animal models. We also tested the effects of combination of several drugs and ADRCs, H2S on angiogenesis in hind limb ischemia model. We have already performed clinical cases using ADRC injection to patients with critical limb ischemia, and examined the possible biomarkers that can predict the salutary efficacy of this procedure. For example, we are focusing on the role of VEGF-A165b and circulating CD34+ cells.

Free Research Field

循環器内科学