Engineeing of the heart tissues using decellularized extracellular matrix - Development of basic technology to construct three-dimensional organ-like tissues

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293188
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Osaka University
• Principal Investigator: LEE JONG-KOOK 大阪大学, 医学(系)研究科(研究院), 特任准教授(常勤) (60303608)
• Co-Investigator(Kenkyū-buntansha): 宮川 繁 大阪大学, 医学(系)研究科(研究院), 特任准教授(常勤) (70544237)
• Research Collaborator: MIWA Keiko 大阪大学, 医学系研究科, 特任助教(常勤) ; YOKOYAMA Teruki 大阪大学, 医学系研究科, 大学院生 ; TOMOYAMA Satoki 大阪大学, 医学系研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ３次元組織構築 / 脱細胞化 / 細胞外マトリックス / 心筋細胞 / iPS細胞 / 光学マッピング / 興奮伝播 / 組織透明化

Outline of Final Research Achievements

Adult rat hearts were decellularized with the perfusion of detergents, and then extracellular matrices were recellularized with cardiac cells from neonatal rats. We evaluated functional and morphological properties of organ-like three dimensional heart tissues using optical mapping and other methods. The results were published in an international journal of biomaterial sciences（Yasui et al. Biomaterials. 2014; IF 8.387）. Furthermore, we succeeded in observing coronary vessels and sympathetic nerves of entire murine hearts using tissue-clearing techniques. The technique can be utilized to evaluate detailed morphological properties of organ-like three-dimensional heart tissues. (Yokoyama et al. Presented in AHA2016 etc. The manuscript is in review.)

Free Research Field

再生医学、組織工学、循環器内科学