Analysis of the mechanisms by which mast cell activation is regulated by paired receptors LMIR3 and LMIR7

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293231
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Juntendo University
• Principal Investigator: Kitaura Jiro 順天堂大学, 医学(系)研究科(研究院), 准教授 (30282651)
• Co-Investigator(Kenkyū-buntansha): 北村 俊雄 東京大学, 医科学研究所, 教授 (20282527)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アレルギー / 炎症 / マスト細胞 / 抑制型受容体 / 活性化型受容体

Outline of Final Research Achievements

LMIR3-deficient colonic mast cells were pivotal in the exacerbation of DSS-induced colitis in LMIR3-deficeint mice. Ceramide vesicles attenuated DSS-induced colitis by inhibiting ATP-mediated activation of colonic mast cells through ceraimide-LMIR3 binding. On the other hand, LMIR3-deficient mice were highly resistant to septic peritonitis induced by cecal ligation and puncture (CLP). Ceramide-LMIR3 binding inhibited the release of neutrophil chemoattractaaants from mast cells and neutrophils in response to E. coli, thereby suppressing neutrophil recruitment to sites of infection. Accordingly, disrupting ceramide-LMIR3 interactions prevents septic peritonitis by stimulating neutrophil recruitment.

Free Research Field

アレルギー・免疫学