Identification of novel biomarkers for pancreato-biliary cancer by comprehensive protein profiling analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293299
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: International University of Health and Welfare (2016); Chiba University (2014-2015)
• Principal Investigator: Miyazaki Masaru 国際医療福祉大学, 大学病院, 教授 (70166156)
• Co-Investigator(Kenkyū-buntansha): 高野 重紹 千葉大学, 医学部附属病院, 助教 (20436380) ; 久保木 知 千葉大学, 医学部附属病院, 助教 (50571410) ; 吉富 秀幸 千葉大学, 医学部附属病院, 講師 (60375631) ; 加藤 厚 千葉大学, 医学(系)研究科(研究院), 講師 (70344984) ; 清水 宏明 千葉大学, 医学(系)研究科(研究院), 准教授 (80272318) ; 大塚 将之 千葉大学, 医学(系)研究科(研究院), 講師 (90334185) ; 賀川 真吾 千葉大学, 医学部附属病院, 助教 (90507302)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 癌 / 外科 / プロテオーム

Outline of Final Research Achievements

We aimed to elucidate the key molecules involved in pancreatic ductal adenocarcinoma (PDAC) progression using proteomics approaches. First, we performed two-dimensional electrophoresis to identify the proteins overexpressed in PDAC tissues. Cofilin-1 were identified and verified as a candidate protein commonly upregulated in PDAC tissues. In immunohistochemistry, samples were divided into two groups based on the level of cofilin-1 expression, the High expression group showed significantly higher incidence of hematogenous dissemination in relapse forms of patients than did the Low expression group. After we established a detection system for immune-complex (IC) of cofilin-1 in sera, we observed that cofilin-1 IC levels in PDAC patients were significantly higher than those in healthy volunteers and patients with pancreatitis. Notably, those levels showed a stepwise increase during PDAC progression. These results suggest that cofilin-1 IC in sera is a potential serum biomarker for PDAC.

Free Research Field

消化器外科