The analysis of inflammasome activation in extracellular histones

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293387
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Osaka Institute of Technology
• Principal Investigator: Kawahara Ko-ihi 大阪工業大学, 工学部, 教授 (10381170)
• Co-Investigator(Kenkyū-buntansha): 丸山 征郎 鹿児島大学, 医歯(薬)学総合研究科, 教授 (20082282) ; 伊藤 隆史 鹿児島大学, 医歯(薬)学総合研究科, 講師 (20381171) ; 森元 陽子 鹿児島大学, 医学部・歯学部附属病院, 助教 (30437967) ; 三浦 直樹 鹿児島大学, 獣医学部, 准教授 (80508036) ; 中島 利博 東京医科大学, 医学部, 教授 (90260752)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 敗血症 / インフラマソーム / ヒストン

Outline of Final Research Achievements

Recently, extracellular histones are damage-associated molecular patterns (DAMPs), which is released by necrotic cells. However, extracellular histones remain unclear as DAMPs. In this study, we focused whether extracellular histones activate inflammasome (IL-1β release) in macrophage cell lines, which are ASC in the presence or absence. Therefore, histones induced IL-1βin macrophage cell-lines in the presence or absence ASC, suggesting that sepsis might enhance in extracellular histones.

Free Research Field

医歯薬学