Regeneration of rat molar pulp tissue by the implantation of stem cells from incisor pulp: development of a rat model of autologous pulp tissue engineering

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293405
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OKIJI Takashi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (80204098)
• Co-Investigator(Kenkyū-buntansha): 吉羽 永子 新潟大学, 医歯学総合病院, 講師 (10323974) ; 吉羽 邦彦 新潟大学, 医歯学系, 准教授 (30220718) ; 大島 勇人 新潟大学, 医歯学系, 教授 (70251824) ; 金子 友厚 新潟大学, 医歯学総合病院, 助教 (70345297)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯学 / 歯内治療学 / 歯髄再生 / 歯髄幹細胞 / 血管内皮細胞

Outline of Final Research Achievements

This study aimed to establish an experimental autologous coronal pulp regeneration model using rat molars and examine whether co-implantation of endothelial cells with stem cells accelerates pulp tissue regeneration. Rat bone marrow mesenchymal stem cells (MSCs) with PLLA/peptide hydrogel constructs were implanted into the coronal pulp chamber of pulpotomized maxillary first molars of Wistar rats. One week after the implantation, a pulp-like tissue was generated in the pulp chamber. In teeth in which rat endothelial cells were co-implanted with MSCs, gene expression levels of pro-angiogenic factors such as Cxcl1 and dentin sialophosphoprotein were elevated compared with MSC-implanted teeth. The co-implanted teeth also showed accelerated scaffold absorption and complete dentin bridge formation.　A similar analysis is in progress using stem cells isolated from rat incisor pulp tissues.

Free Research Field

保存治療系歯学