Trans-differentiation of human fibroblasts into chondrocytes and osteocytes and its application for regenerative medicine

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293419
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IKEDA Masa-Aki 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (20193211)
• Co-Investigator(Kenkyū-buntansha): 池田 やよい 愛知学院大学, 歯学部, 教授 (00202903) ; 大谷 清 関西学院大学, 理工学部, 教授 (30201974) ; 春日井 昇平 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70161049)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨 / 軟骨 / 繊維芽細胞 / 間葉系幹細胞 / 分化転換 / 細胞培養 / 増殖因子 / 再生医療

Outline of Final Research Achievements

The objectives of this study were to determine (1) culture conditions that enable human fibroblasts to trans-differentiate into chondrocytes and osteocytes, and (2) culture conditions that enable mesenchymal stem cells (MSCs) to maintain proliferation and multilineage differentiation potential in vitro long-term culture. We found a combination of culture supplements that enabled to trans-differentiate human dermal fibroblasts into osteocytes and adipocytes, but not chondrocytes. Furthermore, we found a combination of culture supplements that enabled to maintain proliferation potential of bone marrow-derived MSCs (BM-MSCs) in vitro. The long-term cultured MSCs kept osteogenic, adipogenic, and chondrogenic differentiation capacity and expressed the lineage-specific differentiation markers. These results indicate that the extended culture conditions enabled to maintain proliferation capacity of BM-MSCs while retaining their multipotent differentiation capacity.

Free Research Field

歯科医用工学・再生歯学