2017 Fiscal Year Final Research Report
Searching for nitrogen cycling factors at N2O emitting hotspots in different latitude zones and biological agents for rehabilitation of N2O emitting hotspots
| Project/Area Number |
26304042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 海外学術 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宮本 敏澄 北海道大学, 農学研究院, 講師 (00343012)
小林 真 北海道大学, 北方生物圏フィールド科学センター, 助教 (60719798)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | N2O emitter / N2O quencher / degrading boreal peat / Andisol corn farmland / Sphagnum riparium / nitrogen cycle |
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| Outline of Final Research Achievements |
Hyper-N2O emitting microorganisms were screened from Sphagnum riparium, which is tolerant to conditions polluted with minerals and ammonia. According to our N2O emission assay, Rhizobium sp. of class Alphaproteobacteria was isolated as a hyper-active N2O emitting eubacteria. This bacterium formed almost equivalent mixture with a Serratia sp. of class Gammaproteobacteria and administered an ability to produce N2O to the Serratia. In addition, we have isolated non-denitrifying N2O quenchers from both Andisol in Hokkaido and degrading palsa mire in Finland, and the potent N2O quenchers were identified as Rhodococcus sp. and Chitinophaga sp. respectively. Both of the N2O quenchers were missing both nosZ gene for denitrifiers and atypical nosZ genes for dissimilatory nitrite reduction to ammonia (DNRA) bacteria. Hence their N2O quenching mechanisms were not simple reduction of N2O to N2 gas, but more drastic and active catabolic reaction of N2O as their nitrogen source was predicted.
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| Free Research Field |
Environmental Microbiology
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