Basic study on the cellular immunity of alkylbenzene sulfonate as an environmental pollutant

2016 Fiscal Year Final Research Report

• Project/Area Number: 26340027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Takasaki University of Health and Welfare
• Principal Investigator: TANAKA Susumu 高崎健康福祉大学, 健康福祉学部, 教授 (70348142)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: カルシニューリン / アルキルベンゼンスルホン酸 / インターロイキン-2

Outline of Final Research Achievements

We identified that linear alkylbenzene sulfonate (LAS) from acrylonitrile butadiene rubber, recognized as an environmental pollutant, is an inhibitor of calcineurin (CN)-related activity involved in cellular immunity. Studies on inhibition of CN activity using LAS analogues revealed that the inhibitory effect of LAS increased depending on the length of the alkyl chain and the anionic surfactant restored CN inhibition by LAS. Using a double-reciprocal plot, C12-LAS was determined to have noncompetitive inhibitory effects. Subsequently, we assessed the cellular immunity role of LAS by examining the effects of LAS on interleukin-2 (IL-2) produced by Jurkat cells. It was revealed that ConA-induced IL-2 produced by Jurkat cells was suppressed by LAS via the transcription factor NFAT.

Free Research Field

生化学