2017 Fiscal Year Final Research Report
Development of evaluation method using plant callus with a monitoring gene capable of detecting biological effects of the environmental radiation
| Project/Area Number |
26340044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
玉置 雅紀 国立研究開発法人国立環境研究所, 福島支部, 主席研究員 (00311324)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 放射線影響 / 植物 / シロイヌナズナ / カルス / モニター遺伝子 |
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| Outline of Final Research Achievements |
In this study, we developed novel monitoring system to estimate the effect of low-dose radiation originated from contaminated soil by Fukushima-Daiichi Nuclear accident in 2011. We used plant culturing cells (callus) harboring a monitoring gene constructed by alterative GUS gene. In this system, we can detect the frequency of homologous recombination in somatic cells as the radiation-induced DNA damage by counting GUS spots, and then evaluate the effects of low-dose radiation.
We evaluated radiation effects on plant genome with dose-dependent manner both in experimental condition using contaminated soil and in field condition. As a result, we succeeded in generation of an experimental system to detect DNA damage from the low-dose radiation not only in experimental room condition but in field condition.
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| Free Research Field |
環境植物分子生物学
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