2016 Fiscal Year Final Research Report
Development of technology for degradation of formaldehyde in liquid and gas phases using micro-organism
| Project/Area Number |
26340065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental engineering and reduction of environmental burden
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| Research Institution | Wakayama National College of Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Formaldehyde degradation / Methlobacterium sp. FD1 / formaldehyde dismutase / gene cloning / recombinant E. coli / immobilized cells |
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| Outline of Final Research Achievements |
In present study, the formaldehyde dismutase gene (1,203 bp) was cloned from the genomic DNA of Methylobacterium sp.FD1. The resting Escherichia coli DE3 cells that were transformed with the FD1 formaldehyde dismutase gene degraded high concentrations of formaldehyde (2.0%) and produced formic acid and methanol that were molar equivalents of one-half of the degraded formaldehyde. The lyophilized cells of the recombinant E. coli also degraded high concentrations of formaldehyde. Formaldehyde (0.1%) was almost degraded in cloth-immobilized FD1 cell-bioreactor on the continuous operation. Alginate calcium immobilized FD1 cells degraded formaldehyde in gas phase. From the above results, Methylobacterium sp. FD1 cells and E. coli transformed with FD1 formaldehyde dismutase gene are good candidates for agents to degrade formaldehyde in liquid and gas phase.
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| Free Research Field |
応用微生物学
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