2016 Fiscal Year Final Research Report
Elucidation of degradation genes of cold-resistant hydrocarbon degrading bacteria and multifaceted functional analysis of plant growth promotion Rhizospheric bacteria
| Project/Area Number |
26340067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Muroran Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Penicillium / 炭化水素 / 根圏土壌 / 低温域 / 南極 / メタゲノム / アルキルフェーノル |
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| Outline of Final Research Achievements |
Biodegradability of eight different aliphatic and aromatic hydrocarbons (octane, decane, dodecane, ethylbenzene, butylbenzene, naphthalene, acenaphthene, and benzo[a]pyrene) was confirmed using Penicillium sp. CHY - 2 isolated from Antarctic soil. In addition, it was found that the enzyme manganese peroxidase (MnP) derived from strain CHY -2 was involved in the degradation of decane which showed the highest degradation rate among the tested aliphatic and aromatic hydrocarbons. Furthermore, various rhizosphere microorganisms were isolated and found to be involved in hydrocarbon decomposition. As a result of confirming the gene of strain CHY-2 responsible for degradation, genes encoding cytochrome P450 hydroxylase, lignin peroxidase and vanillyl alcohol oxidase were confirmed.
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| Free Research Field |
応用微生物学
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