2016 Fiscal Year Final Research Report
Mechanism of uptake of soil-binding elements by protozoans with symbiotic Chlorella
| Project/Area Number |
26340072
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | セシウム除染 / 原生生物 / 細胞内共生 / 油滴 / EDS |
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| Outline of Final Research Achievements |
Paramecium bursaria (Pb), a protozoan with symbiotic chlorella, dissociates the soil - bound cesium from the soil and accumulates in the cell at high concentration. Element mapping by EDS method was carried out and the migration pathway of cesium in Pb was searched. Cesium was accumulated once in the oil droplet in the symbiotic chlorella in Pb and then transferred to oil droplets in the cytoplasm of Pb. Oil droplets were isolated from Pb and a comprehensive analysis of oil droplet proteins by SDS-PAGE and mass spectrometry was performed. It was also confirmed that over 80% of Pb can be recovered from the soil suspension by prototyping Pb separation and recovery device utilizing Pb galvanotaxis.
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| Free Research Field |
原生生物学
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