Development of hydrogen gas production system from cellulosic biomass by anaerobic bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 26340100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Design and evaluation of sustainable and environmental conscious system
• Research Institution: Mie University
• Principal Investigator: Kimura Tetsuya 三重大学, 生物資源学研究科, 教授 (00281080)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 水素ガス / 嫌気性細菌 / セルロース / 代謝工学 / 微生物遺伝学

Outline of Final Research Achievements

Clostridium paraputrificum isolated from seaside soil is an anaerobic bacterium that produces a large amount of hydrogen gas. Genes encoding lactate dehydrogenase, pyruvate-ferredoxin oxidoreductase, thiolase, phospho-trans-acetylase and phospho-trans-butylase were inactivated by ClosTron method. Each transformant was cultivated anaerobically in 100mL of GS-medium containing glucose as a sole carbon source and hydrogen gas produced during cultivation was analyzed by gas chromatography. The production of the hydrogen gas of the transformant that lacked lactate dehydrogenase became 1.4 times of the wild type strain. Next, we attempted to produce hydrogen gas by C. paraputrificum using cellulose as carbon source. C. paraputrificum cannot use cellulose as a carbon source. Therefore, Ruminiclostridium josui that was isolated from compost was used as cellulose degrader. The mix-culture of both strain was able to produce hydrogen gas from crystalline cellulose.

Free Research Field

環境技術