Establishment of a rapid, simple and ultrasensitive method for detecting chicken infected campylobacter in the field.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Osaka Prefectural Institute of Public Health
• Principal Investigator: Kawatsu Kentaro 大阪府立公衆衛生研究所, 感染症部, 課長 (20260367)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: カンピロバクター / 蛍光イムノクロマト法 / 高感度検出 / 鶏糞便

Outline of Final Research Achievements

We attempted to establish a rapid, simple and ultrasensitive fluorescence immunochromatographic assay for detecting chicken infected campylobacter in the field.
The sensitivities of the experimental fluorescence immunochromatographic kit established in the present study were found to range from 1.1×1000 to 1.7×10000 CFU/ml for C. jejuni and from 1.4×1000 to 1.8×10000 CFU/ml for C. coli, respectively. This kit was more sensitive than a conventional colloidal gold immunochromatographic assay and appeared to be useful for detecting chicken infected campylobacter in the field.

Free Research Field

食品細菌学