2017 Fiscal Year Final Research Report
Development of DNA identification tool in Brassica species applicable to science education in cooperation with school
| Project/Area Number |
26350229
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science education
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| Research Institution | University of Fukui |
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Principal Investigator |
Nishizawa Toru 福井大学, 学術研究院教育・人文社会系部門(教員養成), 准教授 (80414382)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | アブラナ類 / 分子マーカー / DNAマーカー / 生物教材 / 生物教育 / 理科教育 / 理科教材 |
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| Outline of Final Research Achievements |
To capture molecular markers that are applicable to species identification of three Brassica species (B. rapa, B. napus, B. juncea), I screened variations in nucleotide sequences of chloroplast and nuclear DNA. Detection of DNA polymorphisms were analyzed by PCR-RFLPs (Restriction fragment length polymorphisms) and PCR-SSCP (Single strand conformation polymorphisms), but hypervariable loci that enables us to discriminate three Brassica species could not be captured.
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| Free Research Field |
植物系統分類学，生物教育
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