Microbial experiment using insoluble fibroin membrane-immobilized luminescent bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 26350259
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science education
• Research Institution: Oita National College of Technology
• Principal Investigator: Ninomiya Junko 大分工業高等専門学校, 一般科理系, 准教授 (60632726)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 環境教育 / 発光細菌 / 実験教材 / 毒性試験 / フィブロイン

Outline of Final Research Achievements

We tried to prepare an experimental teaching material to prepare an insoluble fibroin film (hereinafter referred to as IFF) and perform toxicity test using a tube in which luminescent bacteria are immobilized on IFF. V. fischeri (ATCC 49387) showing the requirement for sulfur source showed increased emission relative to the linear alkylbenzene sulfonate (LAS) which is a surfactant. When luminescent bacteria were immobilized on IFF, it was revealed that they showed a sharper reaction than the susceptibility test in solution. It was suggested that the toxicity test with luminescent bacteria immobilized on IFF is effective as a new experimental teaching material for environmental education.

Free Research Field

理科教育