Clarification of molecular mechanisms underlying the analgesic effect of botulinum toxin and applied study

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350499
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Okayama University
• Principal Investigator: YAMAMOTO YUMIKO 岡山大学, 医歯薬学総合研究科, 助教 (20403496)
• Co-Investigator(Renkei-kenkyūsha): MATSUSHITA Osamu 岡山大学, 大学院医歯薬学総合研究科, 教授 (00209537)
• Research Collaborator: OGUMA Keiji 岡山大学, 名誉教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ボツリヌス神経毒素 / 神経障害性疼痛 / 疼痛抑制 / SNAP-25

Outline of Final Research Achievements

We developed an assay to measure the proteolytic activity of botulinum toxin (BoNT), and attempted to synthesize fluorescently labeled full-length BoNT, in order to study molecular mechanisms for the analgesic effect of BoNT. We also generated four kinds of anti-peptide antibodies against SNAP-25, and showed that BoNT/A or BoNT/E cleaves SNAP-25 in cultured trigeminal ganglion neurons. This suggests that peripherally administered BoNT decreases the release of neurotransmitters via the proteolysis of SNARE proteins in sensory ganglion neurons and relieves neuropathic pain.

Free Research Field

細菌学