2016 Fiscal Year Final Research Report
Quantitative evaluation of the change in action potential and axonal injury in brain neurons exposed to impulsive strain
| Project/Area Number |
26350509
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 頭部外傷 / 軸索損傷 / ひずみ / ひずみ速度 / 軸索伸長方向制御 / Axonal swellings / Axonal bulb / Tauタンパク質 |
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| Outline of Final Research Achievements |
Axonal injury is caused by sudden inertial loading to the head associated with rapid deformation of brain tissue, resulting in the stretching of neural axons. This study aims to clarify the relation between axonal stretch and the onset of axonal injury. To examine the influence of strain rate on the threshold for axonal injury, cultured neurons were subjected to uniaxial stretching. Axonal injury increased significantly more than strain rate of 30 /s with more than strain of 0.15 even though those formations did not depend on strain rates in cultures exposed to a strain of 0.10. To examine the influence of the strain along an axon on axonal injury axonal injury was measured at every axonal angle in the stretching direction. The axons that were parallel to stretching direction were injured the most. We proposed an experimental model that subjected an axon to more accurate strain for the measurement of action potential in single neuron before and after stretching.
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| Free Research Field |
バイオメカニクス
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