2016 Fiscal Year Final Research Report
Development of high contrast fluorescence microscopy using enhanced fluorescence of an optical interference mirror slide
| Project/Area Number |
26350518
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo University of Technology |
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Principal Investigator |
Akimoto Takuo 東京工科大学, 応用生物学部, 教授 (90367194)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | バイオセンサー / 蛍光増強 / 蛍光顕微鏡 |
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| Outline of Final Research Achievements |
An enhanced fluorescence can be observed with an optical interference mirror slide consisting of a metal and a dielectric layer. However, a general fluorescence microscopy does not provide the enhanced fluorescence because of the polarization dependence of the enhanced fluorescence. To obtain the enhanced fluorescence, we developed a fluorescence microscope equipped with a concentric circular polarized excitation. As a result, 42-fold enhanced fluorescence was achieved by the microscope with the concentric circular polarized excitation.
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| Free Research Field |
バイオセンサー
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