Arrhythmogenic Drug Evaluation System by Simplified Animal Experiments

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Ritsumeikan University
• Principal Investigator: Amano Akira 立命館大学, 生命科学部, 教授 (60252491)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 薬物誘発性不整脈 / 活動電位波形 / 心筋細胞モデル / イオンチャネル / HERG

Outline of Final Research Achievements

In this research, we developed a tool to evaluate arrhythmogenic effect of drugs which is one of the most problem in drug discovery, by using cardiac cell model parameter optimization. In the system, the cardiac action potential is first measured by open chest guinea pig suction electrode. Then the action potential shape is reproduced with cardiac cell model by optimizing the ion channel permeability parameters. Finally, the changes in the ion channel permeability is used to estimate drug effect to the ion channels. From the experimental results, the drug inhibition to the ion channels of IKr, IKs and ICaL could be estimated by certain extent.

Free Research Field

生体機能シミュレーション