Assessment of Whole Blood Rheology and Leukocyte Activity in ex vivo Microchannel Array Model to Contribute Cardiovascular Rehabilitation Research

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350581
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Dokkyo Medical University
• Principal Investigator: YASU TAKANORI 獨協医科大学, 医学部, 教授 (40265278)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 微小循環 / 血液流動性 / リハビリテーション / 運動 / 抹消動脈疾患 / 白血球

Outline of Final Research Achievements

Leucocytes play an important role in the all process of the atherosclerosis. However, clinically feasible parameters of leukocyte activation in microcirculation is limited. We developed a clinically feasible assessment method of leukocyte activity and blood rheology using a microchannel flow analyzer (MCFAN) with new designed silicon chips mimic to human microvascular network. The passage time of whole blood with heparin or EDTA-2Na+heparin through the microchannel arrays was measured using MC-FAN. Number of adhesive leukocytes (/field) was counted in offline as a gold standard of leukocyte activation. Subtraction of the passage time of blood with heparin and one with EDTA-2Na + heparin was significantly correlated with adhesive leukocyte number. The delta whole blood passage time may be a novel parameter reflecting leukocyte activity.

Free Research Field

人間医工学