2016 Fiscal Year Final Research Report
Investigation of new zinc finger protein function as a redox sensor for reactive oxygen in the cells
| Project/Area Number |
26350962
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Doshisha Women's College of Liberal Arts |
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Principal Investigator |
NEGI SHIGERU 同志社女子大学, 薬学部, 准教授 (50378866)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 亜鉛フィンガータンパク質 / レドックス反応 / レドックスセンサー / DAN結合 |
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| Outline of Final Research Achievements |
In this study, we tried to investigate the oxidation reactivity, stability of the folding structure, DNA-binding property using Sp1 zinc finger protein.
As a result of an oxidation reaction with H2O2, reactivity became the following order, F1>>F2>F3, indicating that each three finger has a non-equivalence reactivity for the oxidation reaction. In DNA-binding assay using the gel shift method, oxidized Sp1 zinc finger protein lost the DNA-binding ability due to Zn(II) ion release following S-S bond formation.
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| Free Research Field |
複合新領域 生物分子化学
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