2016 Fiscal Year Final Research Report
Fanctional analysis of diterpene synthase responsible for fungal conidiation Inducing and its diterpene reseptor discovery
| Project/Area Number |
26350965
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ジテルペン / 環化酵素 / 真菌 / 分生子 / 阻害剤 / ドッキングシミュレーション / Penicillium / Aspergillus |
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| Outline of Final Research Achievements |
Recently, the fungus Penicillium cyclopium was found to produce conidiogenol and conidiogenone as self conidiation inducing-diterpenoids. This finding indicates that a novel diterpene synthase would utilize for the biosynthesis of these diterpenoids. To study the conidiation in fungi, we have identified two novel diterpene synthases (PeDS and AnDS1) from P. cyclopium synonym and Aspergillus nidulans A4. Heterologous expressed in vivo enzyme assays revealed that PeDS catalyzes the GGDP synthesis and convert into novel 3-deoxyconidiogenol, and AnDS1 catalyzes the GGDP synthesis and into novel diterpene hydrocarbon, respectivery. The structures of two novel compounds were elucidated by spectral data. These compounds were revealed to have conidiation inducing activities in the submerged culture of P. cyclopium synonym and A. nidulans A4, respectivery.
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| Free Research Field |
天然物化学、生物分子科学
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