Creation of diversified molecular formations and functions using artificial cross-linking and non-natural amino acids

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350970
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Saitama University
• Principal Investigator: NEMOTO Naoto 埼玉大学, 理工学研究科, 教授 (60509727)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 進化工学 / ペプチドライブラリ / 多様性 / 試験管内進化

Outline of Final Research Achievements

cDNA display is an in vitro selection method to evolve peptides and proteins, which allow the introductions of non-natural amino acids and chemical modification after translation. This advantage enables a peptide to form a multiple of formation. In this study, we developed two kinds of elemental technologies to diversify peptide formations, 1) introduction of non-natural amino acids, 2) chemical cross-linking of disulfide bonds in a peptide with cross-linking agents. These achievements were published by seven journal papers and three patents.

Free Research Field

進化分子工学