Radiosensitization effect of liposome-encapsulated fullerenes on human melanoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 26390017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials chemistry
• Research Institution: Mie University
• Principal Investigator: Kato Shinya 三重大学, 地域イノベーション推進機構, 助教 (40545547)
• Co-Investigator(Kenkyū-buntansha): 吉村 哲郎 三重大学, 工学研究科, 特任教授(研究担当) (30035472)
• Research Collaborator: MIWA Nobuhiko 県立広島大学, 名誉教授
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: リポソーム / フラーレン / 脂質過酸化

Outline of Final Research Achievements

The polyhydroxy small-gap fullerenes (SGFs: C120O30(OH)30・30H2O・25Na+) were encapsulated in MLV liposomes (Lpsm) composed of DOPC and DOPS, which are designated as LpsmSGFs (DOPC/DOPS/SGFs = 35 mM:15 mM:246-445 μM, φ141.2 nm, ζ-potential-35.65 mV). Radiosensitization by LpsmSGFs under X-ray irradiation was evaluated on human melanoma HMV-II cells. On 7th day after X-ray irradiation, cell proliferation degree decreased on cells pretreated with LpsmSGFs than Lpsm or free-SGFs. LpsmSGFs obviously exhibited more augmented mitochondrial membrane potentials on perinuclear region of cells than Lpsm or free-SGFs. The oxidation-reduction potentials of SGFs aqueous solution increased by X-ray irradiation. These results suggest that LpsmSGFs-mediated generation of reactive oxygen species results in damages to cellular components such as mitochondria and DNA on cells, and thereby cell proliferation decreased. The LpsmSGFs has a potential as a pro-oxidative type radiosensitizer.

Free Research Field

応用生物化学