Development of novel techniques for single-molecule fluorescence imaging of various biomolecules

2016 Fiscal Year Final Research Report

• Project/Area Number: 26390035
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanobioscience
• Research Institution: Waseda University
• Principal Investigator: TANII Takashi 早稲田大学, 理工学術院, 教授 (20339708)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 1分子イメージング / 蛍光観察 / FRET / ナノ構造

Outline of Final Research Achievements

In order to enhance the ability of single-molecule fluorescence imaging (SMFI) that enables to detect binding and dissociation events of bio-molecules in real time using zero-mode waveguides (ZMWs), we propose a novel technique to combine fluorescence resonance energy transfer (FRET) and SMFI with ZMWs, and proved experimentally that the proposed method is feasible. We also tried to extend the ability of this technique by demonstrating SMFI of individual DNA aptamers for sensing application, SMFI of DNA strand scission by endonuclease, and in-situ cell patterning.

Free Research Field

分子ナノ工学