Non-contact, non-staining three-dimensional microscopic imaging based on high-precision phase measurement

2017 Fiscal Year Final Research Report

• Project/Area Number: 26390079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Optical engineering, Photon science
• Research Institution: The University of Electro-Communications
• Principal Investigator: Watanabe Eriko 電気通信大学, 大学院情報理工学研究科, 准教授 (20424765)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 光干渉計測 / ホログラフィ

Outline of Final Research Achievements

In this research, we developed a quantitative optical path length difference and polarization microscopy (QPPM) and a digital holographic microscope (DHM). The QPPM was accomplished by introducing a polarimetric module to one arm of the Mach-Zehnder interferometer in our conventional phase imaging system. The QPPM can almost simultaneously measure the optical path length difference, birefringence phase difference, and the azimuth angle of birefringence, using our new base-correction method, which reduced the influence of elements other than cells. We also demonstrated the high-precision three-dimensional microscopic imaging of three-dimensionally arranged beads and living cells using our developed DHM. This DHM was completed by introducing a design method for an optical system that accurately acquires intensity distribution and phase distribution, and by improving the phase shift method and the three-dimensional data correction method.

Free Research Field

光情報処理