2016 Fiscal Year Final Research Report
Elucidation of DNA higher order conformation by development of a novel tweezer controlling force and torque independently
| Project/Area Number |
26400426
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological physics/Chemical physics/Soft matter physics
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Murayama Yoshihiro 東京農工大学, 工学(系)研究科(研究院), 准教授 (60334249)
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| Research Collaborator |
ANDO Daisuke
YOSHIDA Kotaro
KURODA Masafumi
ABE Hiroki
MASUDA Shinichiro
TANOGUCHI Masaya
SAGA Sayuka
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | DNA / 高次構造 / 構造変化 / 捩じれ / 張力 / トルク |
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| Outline of Final Research Achievements |
Complicated conformations of DNA appear when strain is accumulated by stretching and twisting DNA. In living cells, gene expression is regulated by regulating the higher order conformation of DNA. In this study, we have developed a novel tweezer which enables us to stretch and twist a single DNA independently, and succeeded to observe the conformational change in low force regime that is hard to access by traditional method. Our results indicate that not only a protein design but also conformational design can be coded in DNA base sequence.
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| Free Research Field |
生物物理学
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