Formation of RecA helical structures on hybrid surfaces of DNA and carbon nanotubes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26400436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Tokyo University of Science
• Principal Investigator: UMEMURA KAZUO 東京理科大学, 理学部第二部物理学科, 教授 (60281664)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: DNA / RecA / DNA結合蛋白質 / カーボンナノチューブ

Outline of Final Research Achievements

DNA binding proteins such as RecA proteins and single-stranded DNA binding (SSB) proteins were reacted with various carbon nanotube (CNT) surfaces that were wrapped with DNA and polymers in order to study bio-recognition functions of the protein molecules and interactions among the molecules. Styles of protein adsorption and binding was significantly changed due to surface conditions of CNTs. Especially, two different interactions were competitive. One is interactions between proteins and DNA, and the other is those between proteins and CNTs. This suggests that interactions of DNA and DNA binding proteins are well controlled. In addition, regulation of the two interactions is probably one of the important factors for developing nanobiodevices using CNTs.

Free Research Field

生物物理学