Structural Study of protein A and its derivatives in solution

2016 Fiscal Year Final Research Report

• Project/Area Number: 26400438
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Himeji Hinomoto College
• Principal Investigator: Kihara Hiroshi 姫路日ノ本短期大学, その他部局等, 特任教授 (20049076)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ｘ線溶液散乱 / プロテインA / 蛋白質の溶液内構造 / 免疫グロブリン

Outline of Final Research Achievements

Protein A is oftein used for preparation of immunoglobulins. However, its conformation is solution has not known. We have used X-ray solution scattering method for obtaining the conformation of protein A and its derivatives.
Results are also compared with the structure predicted by molecular dynamics, which gives the very good coincidence. This result suggests the protein A takes the relaxed conformaton without the stress.
We have also done X-ray solution scattering study on the complex of protein A and Fc fragment of Immunoglobulin, and successfully obtained the scattering profile of the complex. Further analysis should be done.

Free Research Field

X線溶液散乱